A conditional knockout-rescue system was developed to construct an in-frame deletion strain topo designs cooler bag of Escherichia coli essential genes.The target was flanked with marker genes and FRT (FLP recognition target) sites, and a plasmid containing arabinose-induced FLP recombinase was transformed.After arabinose induction, cells could sur
Purpose: This paper aims to examine the occupational structures of selected EU member countries and assess their compatibility with the labour market demands, aiming to identify potential structural mismatches.The search evaluates the alignment between existing knowledge and skills in specific qualifications and those required by employers, utilizi
When people make decisions about sequentially presented items in psychophysical experiments, their decisions are always biased by their preceding decisions and the preceding items, either by assimilation (shift towards the decision or item) or contrast (shift away from the decision or item).Such sequential biases also occur in naturalistic and ipho
Considering the risk aversion for gains and the risk seeking for losses of venture capitalists, the TODIM has been chosen as the decision-making method.Moreover, group decision is an available way to avoid the limited ability and knowledge etc.of venture capitalists.Simultaneously, venture capitalists may be hesitant among several assessed values w